PMC

Compounds obtained from A. vineale leaf extract using different isolation methods. (126) 2-furaldehyde; (127) (2E)-hexenal; (128) (3Z)-hexenol; (129) 2,4-dimethylthiophene; (130) allyl methyl disulfide; (131) methyl (Z)-1-propenyl disulfide; (132) methyl (E)-1-propenyl disulfide; (133) benzaldehyde; (134) dimethyl trisulfide; (135) diallyl disulfide; (136) allyl (Z)-1-propenyl disulfide; (137) allyl (E)-1-propenyl disulfide; (138) 1-propenyl propyl disulfide; (139) methyl (methylthio)methyl disulfide; (140) allyl methyl trisulfide; (141) 4-methyl-1,2,3-trithiolane; (142) methyl propyl trisulfide; (143) methyl (Z)-1-propenyl trisulfide; (144) methyl (E)-1-propenyl trisulfide; (145) dimethyl tetrasulfide; (146) allyl (methylthio)methyl disulfide; (147) diallyl trisulfide; (148) allyl (Z)-1-propenyl trisulfide; (149) p-vinylguaiacol; (150) allyl propyl trisulfide; (151) 5-methyl-1,2,3,4-tetrathiane; (152) methyl (methylthio)methyl trisulfide; (153) allyl methyl tetrasulfide; (154) allyl (methylthio)methyl trisulfide; (155) 4-methyl-1,2,3,5,6-pentathiepane []; (156) chrysoeriol; (157) chrysoeriol-7-O[200-O-E-feruloyl]-β-D-glucoside; (158) isorhamnetin-3-O-β-D-glucoside [].

Dual-pulse ion-neutral reaction between benzene radical cation and 1,5-hexadiene (A), or allyl radical (B). Reaction of allyl cation (generated from allyl-bromide) with neutral benzene (C). Reaction of d₆-benzene radical cation and allyl radical (D). Allyl radical was generated by pyrolysis of 1,5-hexadiene.

Allyl GSL accumulation. (A) The average of exogenous allyl GSL accumulation within leaves of 15-day-old seedlings was directly measured using HPLC. Shown are the accessions that are genetically incapable of synthesizing endogenous allyl GSL. Thus, detected allyl must have been taken up from the surrounding media, transported via the vasculature to the leaf and provides a direct measurement of the accumulation. Averages with the same letter are not significantly different at P ≤ 0.05 using a Tukey's post hoc t-test within the ANOVA. The error bars represent standard deviation from three independent experiments where 12 plants were separately measured per experiment. (B) Average of the total endogenous aliphatic GSL and accumulated allyl content within the accessions that do not synthesize endogenous allyl GSL grown in MS with allyl at 1% of sucrose on the growing media. The error bars represent standard deviation across accessions and experiments as described in part (A).